The blots were incubated at 4 overnight?C with primary antibody followed by incubation with anti-rabbit-HRP or anti-mouse-HRP secondary antibody in 5% BSA for 2?h. T cells (Tregs) are one of the subsets of CD4+ T helper cells that are necessary for immune homeostasis. Apart from their roles in maintaining U-69593 … Continue reading The blots were incubated at 4 overnight?C with primary antibody followed by incubation with anti-rabbit-HRP or anti-mouse-HRP secondary antibody in 5% BSA for 2?h